Detailed information of TA system TA038246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 874849..875502
• Replicon: chromosome
• Accession: NZ_AP024259
• Organism: Mycobacterium paraintracellulare strain M021

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN177_RS04465
• Protein ID: WP_008253911.1
• Coordinates: 875041..875502 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: I2A997
• Locus tag: JN177_RS04460
• Protein ID: WP_008253910.1
• Coordinates: 874849..875037 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN177_RS04450	870821..872398	+	1578	WP_202377333.1	serine hydrolase	-
JN177_RS04455	872395..874782	+	2388	WP_014711030.1	cation-translocating P-type ATPase	-
JN177_RS04460	874849..875037	+	189	WP_008253910.1	antitoxin	Antitoxin
JN177_RS04465	875041..875502	+	462	WP_008253911.1	SRPBCC family protein	Toxin
JN177_RS04470	875587..876357	+	771	WP_008253913.1	VOC family protein	-
JN177_RS04475	876431..876865	+	435	WP_008253914.1	hypothetical protein	-
JN177_RS04480	876870..877358	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
JN177_RS04485	877358..878884	-	1527	WP_202376915.1	carotenoid oxygenase family protein	-
JN177_RS04490	878886..880106	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T38246 WP_008253911.1 NZ_AP024259:875041-875502 [Mycobacterium paraintracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T38246 NZ_AP024259:875041-875502 [Mycobacterium paraintracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TTGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6797.44 Da
• Isoelectric Point: 4.4702

>AT38246 WP_008253910.1 NZ_AP024259:874849-875037 [Mycobacterium paraintracellulare]
MGFLDKAKDLLWQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT38246 NZ_AP024259:874849-875037 [Mycobacterium paraintracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTGGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7TPT2