Detailed information of TA system TA038235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1150667..1151231
• Replicon: chromosome
• Accession: NZ_AP024255
• Organism: Mycobacterium intracellulare strain M018

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IVI4
• Locus tag: JN181_RS05745
• Protein ID: WP_009953437.1
• Coordinates: 1150860..1151231 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN181_RS05740
• Protein ID: WP_026071432.1
• Coordinates: 1150667..1150873 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN181_RS05720	1146288..1147286	+	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN181_RS05725	1147332..1148471	-	1140	WP_179293415.1	hypothetical protein	-
JN181_RS05730	1148613..1149686	+	1074	WP_009953440.1	redox-regulated ATPase YchF	-
JN181_RS05735	1149766..1150428	-	663	WP_014379386.1	methyltransferase domain-containing protein	-
JN181_RS05740	1150667..1150873	+	207	WP_026071432.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN181_RS05745	1150860..1151231	+	372	WP_009953437.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN181_RS05750	1151289..1151840	+	552	WP_009953436.1	VOC family protein	-
JN181_RS05755	1151919..1152317	+	399	WP_009953435.1	VOC family protein	-
JN181_RS05760	1152416..1152799	+	384	WP_026071433.1	hypothetical protein	-
JN181_RS05765	1152885..1153244	+	360	WP_179293417.1	hypothetical protein	-
JN181_RS05770	1153426..1154001	+	576	WP_085977620.1	C40 family peptidase	-
JN181_RS05775	1154364..1154687	+	324	WP_009953431.1	antibiotic biosynthesis monooxygenase	-
JN181_RS05780	1154736..1155419	-	684	WP_014384004.1	hypothetical protein	-
JN181_RS05785	1155566..1155775	+	210	WP_014381976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13868.20 Da
• Isoelectric Point: 6.8398

>T38235 WP_009953437.1 NZ_AP024255:1150860-1151231 [Mycobacterium intracellulare]
VILVDTSIWIDHLHVAEQRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSHLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACKEVGVAIVDES

Nucleotide

• Length: 372 bp

>T38235 NZ_AP024255:1150860-1151231 [Mycobacterium intracellulare]
GTGATCCTCGTTGACACATCGATATGGATTGACCATCTGCACGTCGCCGAGCAGCGCCTCGTCGGCCGCTTGACCAACGA
CGAAATCGGTTGCCACCAAATGGTGATCGAGGAGCTGGCACTCGGTATGATCAAGCAGCGCGACGACGTACTGAGATTGC
TGTCCCATCTGCGCGAGTTTCCGTTGTTGACGCACGCGGAAATCCTCCATCTCGTTGACCGTCGCCGCTTGTGGGGAAGG
GGATTGAGTGCAATCGACGCCCACCTTCTTGGGTCGGTTGCTGTCGTCGACGGTGCACGGCTTTGGACGAGGGACAAGAA
GTTGAAAGCGGCGTGTAAAGAAGTGGGTGTTGCGATCGTCGATGAGTCGTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7250.31 Da
• Isoelectric Point: 11.9169

>AT38235 WP_026071432.1 NZ_AP024255:1150667-1150873 [Mycobacterium intracellulare]
MRTTVTIDDALLAQAAELTGVTESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDPR

Nucleotide

• Length: 207 bp

>AT38235 NZ_AP024255:1150667-1150873 [Mycobacterium intracellulare]
ATGAGGACAACAGTGACCATCGACGATGCCTTACTGGCTCAGGCGGCGGAACTGACCGGCGTGACGGAGAGTGCGGCGCT
TCTCCGTCAGGGATTGCAGACCCTCATTCGGGTAGAGAGCGCCCGCCGTCTCGCGGCGCTCGGCGGCACGGACCCCAAGG
CGTCGGCCGCCCCGCGGCGGAGGCCGCCGACCCGTGATCCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A7R7MQX9