Detailed information of TA system TA038232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2275502..2276166
• Replicon: chromosome
• Accession: NZ_AP024254
• Organism: Mycobacterium intracellulare strain M016

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J9WD27
• Locus tag: JN212_RS10810
• Protein ID: WP_009954498.1
• Coordinates: 2275759..2276166 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN212_RS10805
• Protein ID: WP_009954496.1
• Coordinates: 2275502..2275762 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN212_RS10780	2272010..2272501	-	492	WP_014379893.1	DUF1707 domain-containing protein	-
JN212_RS10785	2272571..2273167	+	597	WP_009954492.1	TetR/AcrR family transcriptional regulator	-
JN212_RS10790	2273172..2274440	-	1269	WP_009954493.1	cytochrome P450	-
JN212_RS10795	2274601..2275053	+	453	WP_029384640.1	VOC family protein	-
JN212_RS10800	2275090..2275379	+	290	Protein_2127	hypothetical protein	-
JN212_RS10805	2275502..2275762	+	261	WP_009954496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JN212_RS10810	2275759..2276166	+	408	WP_009954498.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN212_RS10815	2276344..2279445	+	3102	WP_009954499.1	DEAD/DEAH box helicase	-
JN212_RS10820	2279507..2281072	-	1566	WP_009954500.1	serine/threonine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14293.28 Da
• Isoelectric Point: 4.2772

>T38232 WP_009954498.1 NZ_AP024254:2275759-2276166 [Mycobacterium intracellulare]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPVDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T38232 NZ_AP024254:2275759-2276166 [Mycobacterium intracellulare]
ATGAGCAACCCGCAGGTGGCCGGAGTGCTCGACACTTCGGTGTTCATCGCGACCGAAAGTGGTCGGCCTCTCGACGCGGC
GCTGATCCCTGATCAAGTTGGCACTACGGTTGTTACCCTCGCGGAACTGCACGTTGGTGTGTTGGCCGCGTCGACTTCCG
ATATTAGAGCGCAGCGCCTGGCGACATTGGAATCCATTGCGGATATCGAAGCGCTGCCGGTCGATGACGATGCGGCCCGG
ATGTGGGCCCGGCTGCGGATCCATCTCGCCGAGACCGGTCAACGCGTCCGGATCAACGATCTGTGGATCGCGGCCATCGC
GGCGTCCCGAGGGCTGCCTGTTGTCACGCAGGACGATGACTTTGCCGTCTTGGATGGTGCGGCCAATTTAACCGTTATCC
GCGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT38232 WP_009954496.1 NZ_AP024254:2275502-2275762 [Mycobacterium intracellulare]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT38232 NZ_AP024254:2275502-2275762 [Mycobacterium intracellulare]
ATGTCTGAAGTGGCATCTCGTGAGCTGCGCAACGATACGGCTGGGGTGCTGCGGCGGGTTCGGGATGGAGAGGACGTCAC
CATCACTGTCAGTGGCCGACCGGTCGCAGTTCTTACCGCAGTTGTCCCTCAGCGCAGGCGTTGGCTGAGCAAGACGGAGT
TGCTGTCGCGCTTGCCCCGCGTTCAAGCGGATCCGGGATTACGCGAGGACCTTCACGCACTCGCCGGCGACACTACCGAG
GAGCTGGGCCCGATCCGATGA

Structures

Toxin

• AlphaFold DB: J9WD27