Detailed information of TA system TA038231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1140515..1141079
• Replicon: chromosome
• Accession: NZ_AP024254
• Organism: Mycobacterium intracellulare strain M016

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IVI4
• Locus tag: JN212_RS05700
• Protein ID: WP_009953437.1
• Coordinates: 1140708..1141079 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN212_RS05695
• Protein ID: WP_026071432.1
• Coordinates: 1140515..1140721 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN212_RS05675	1136136..1137134	+	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN212_RS05680	1137180..1138319	-	1140	WP_179300624.1	hypothetical protein	-
JN212_RS05685	1138461..1139534	+	1074	WP_009953440.1	redox-regulated ATPase YchF	-
JN212_RS05690	1139614..1140276	-	663	WP_014379386.1	methyltransferase domain-containing protein	-
JN212_RS05695	1140515..1140721	+	207	WP_026071432.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN212_RS05700	1140708..1141079	+	372	WP_009953437.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN212_RS05705	1141137..1141688	+	552	WP_009953436.1	VOC family protein	-
JN212_RS05710	1141767..1142165	+	399	WP_009953435.1	VOC family protein	-
JN212_RS05715	1142264..1142647	+	384	WP_026071433.1	hypothetical protein	-
JN212_RS05720	1142733..1143092	+	360	WP_198139476.1	hypothetical protein	-
JN212_RS05725	1143274..1143849	+	576	WP_085977620.1	C40 family peptidase	-
JN212_RS05730	1144212..1144535	+	324	WP_009953431.1	antibiotic biosynthesis monooxygenase	-
JN212_RS05735	1144584..1145267	-	684	WP_014384004.1	hypothetical protein	-
JN212_RS05740	1145414..1145623	+	210	WP_014381976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13868.20 Da
• Isoelectric Point: 6.8398

>T38231 WP_009953437.1 NZ_AP024254:1140708-1141079 [Mycobacterium intracellulare]
VILVDTSIWIDHLHVAEQRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSHLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACKEVGVAIVDES

Nucleotide

• Length: 372 bp

>T38231 NZ_AP024254:1140708-1141079 [Mycobacterium intracellulare]
GTGATCCTCGTTGACACATCGATATGGATTGACCATCTGCACGTCGCCGAGCAGCGCCTCGTCGGCCGCTTGACCAACGA
CGAAATCGGTTGCCACCAAATGGTGATCGAGGAGCTGGCACTCGGTATGATCAAGCAGCGCGACGACGTACTGAGATTGC
TGTCCCATCTGCGCGAGTTTCCGTTGTTGACGCACGCGGAAATCCTCCATCTCGTTGACCGTCGCCGCTTGTGGGGAAGG
GGATTGAGTGCAATCGACGCCCACCTTCTTGGGTCGGTTGCTGTCGTCGACGGTGCACGGCTTTGGACGAGGGACAAGAA
GTTGAAAGCGGCGTGTAAAGAAGTGGGTGTTGCGATCGTCGATGAGTCGTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7250.31 Da
• Isoelectric Point: 11.9169

>AT38231 WP_026071432.1 NZ_AP024254:1140515-1140721 [Mycobacterium intracellulare]
MRTTVTIDDALLAQAAELTGVTESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDPR

Nucleotide

• Length: 207 bp

>AT38231 NZ_AP024254:1140515-1140721 [Mycobacterium intracellulare]
ATGAGGACAACAGTGACCATCGACGATGCCTTACTGGCTCAGGCGGCGGAACTGACCGGCGTGACGGAGAGTGCGGCGCT
TCTCCGTCAGGGATTGCAGACCCTCATTCGGGTAGAGAGCGCCCGCCGTCTCGCGGCGCTCGGCGGCACGGACCCCAAGG
CGTCGGCCGCCCCGCGGCGGAGGCCGCCGACCCGTGATCCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A7R7MQX9