Detailed information of TA system TA038230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 889579..890232
• Replicon: chromosome
• Accession: NZ_AP024254
• Organism: Mycobacterium intracellulare strain M016

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN212_RS04545
• Protein ID: WP_008253911.1
• Coordinates: 889771..890232 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: JN212_RS04540
• Protein ID: WP_009957321.1
• Coordinates: 889579..889767 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN212_RS04530	885552..887129	+	1578	WP_009957319.1	serine hydrolase	-
JN212_RS04535	887126..889513	+	2388	WP_009957320.1	cation-translocating P-type ATPase	-
JN212_RS04540	889579..889767	+	189	WP_009957321.1	antitoxin	Antitoxin
JN212_RS04545	889771..890232	+	462	WP_008253911.1	SRPBCC family protein	Toxin
JN212_RS04550	890317..891087	+	771	WP_009957322.1	VOC family protein	-
JN212_RS04555	891161..891595	+	435	WP_008253914.1	hypothetical protein	-
JN212_RS04560	891600..892088	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
JN212_RS04565	892088..893614	-	1527	WP_009957325.1	carotenoid oxygenase family protein	-
JN212_RS04570	893616..894836	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T38230 WP_008253911.1 NZ_AP024254:889771-890232 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T38230 NZ_AP024254:889771-890232 [Mycobacterium intracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TTGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT38230 WP_009957321.1 NZ_AP024254:889579-889767 [Mycobacterium intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT38230 NZ_AP024254:889579-889767 [Mycobacterium intracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTCGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCGGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: X8A187