Detailed information of TA system TA038227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1301202..1301766
• Replicon: chromosome
• Accession: NZ_AP024253
• Organism: Mycobacterium paraintracellulare strain M015

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J9WD68
• Locus tag: JN176_RS06380
• Protein ID: WP_014941408.1
• Coordinates: 1301395..1301766 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN176_RS06375
• Protein ID: WP_026071432.1
• Coordinates: 1301202..1301408 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN176_RS06355	1296817..1297815	+	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN176_RS06360	1297861..1299006	-	1146	WP_193375537.1	hypothetical protein	-
JN176_RS06365	1299148..1300221	+	1074	WP_014941407.1	redox-regulated ATPase YchF	-
JN176_RS06370	1300301..1300963	-	663	WP_014384000.1	methyltransferase domain-containing protein	-
JN176_RS06375	1301202..1301408	+	207	WP_026071432.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN176_RS06380	1301395..1301766	+	372	WP_014941408.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN176_RS06385	1301824..1302375	+	552	WP_038535279.1	VOC family protein	-
JN176_RS06390	1302454..1302852	+	399	WP_009953435.1	VOC family protein	-
JN176_RS06395	1302999..1303334	+	336	WP_014379387.1	hypothetical protein	-
JN176_RS06400	1303420..1303779	+	360	WP_179960061.1	hypothetical protein	-
JN176_RS06405	1303961..1304536	+	576	WP_085977620.1	C40 family peptidase	-
JN176_RS06410	1304899..1305222	+	324	WP_009953431.1	antibiotic biosynthesis monooxygenase	-
JN176_RS06415	1305271..1305954	-	684	WP_014384004.1	hypothetical protein	-
JN176_RS06420	1306101..1306310	+	210	WP_014381976.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13868.20 Da
• Isoelectric Point: 6.8398

>T38227 WP_014941408.1 NZ_AP024253:1301395-1301766 [Mycobacterium paraintracellulare]
VILVDTSIWIDHLHVAEHRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSQLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACKEVGVAIVDES

Nucleotide

• Length: 372 bp

>T38227 NZ_AP024253:1301395-1301766 [Mycobacterium paraintracellulare]
GTGATCCTCGTTGACACATCGATATGGATTGACCATCTGCACGTCGCCGAGCATCGCCTCGTCGGCCGCTTGACCAACGA
CGAAATCGGTTGCCACCAAATGGTGATCGAGGAGCTGGCACTCGGTATGATCAAGCAGCGCGACGACGTACTGAGATTGC
TGTCCCAGCTGCGCGAGTTTCCGTTGTTGACGCACGCGGAAATCCTCCATCTCGTTGACCGTCGCCGCTTGTGGGGAAGG
GGATTGAGCGCAATCGACGCCCACCTTCTTGGGTCGGTTGCTGTCGTCGACGGTGCACGGCTTTGGACGAGGGACAAGAA
GTTGAAAGCGGCGTGTAAAGAAGTGGGTGTTGCGATCGTCGATGAGTCGTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7250.31 Da
• Isoelectric Point: 11.9169

>AT38227 WP_026071432.1 NZ_AP024253:1301202-1301408 [Mycobacterium paraintracellulare]
MRTTVTIDDALLAQAAELTGVTESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDPR

Nucleotide

• Length: 207 bp

>AT38227 NZ_AP024253:1301202-1301408 [Mycobacterium paraintracellulare]
ATGAGGACAACAGTGACCATCGACGATGCCTTACTGGCTCAGGCGGCGGAACTGACCGGCGTGACGGAGAGTGCGGCGCT
TCTCCGTCAGGGATTGCAGACCCTCATTCGGGTAGAGAGCGCCCGCCGTCTCGCGGCGCTCGGCGGCACGGACCCCAAGG
CGTCGGCCGCCCCGCGGCGGAGGCCGCCGACCCGTGATCCTCGTTGA

Structures

Toxin

• AlphaFold DB: J9WD68