Detailed information of TA system TA038223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1052322..1052975
• Replicon: chromosome
• Accession: NZ_AP024251
• Organism: Mycobacterium paraintracellulare strain M011

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN179_RS05260
• Protein ID: WP_008253911.1
• Coordinates: 1052514..1052975 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: I2A997
• Locus tag: JN179_RS05255
• Protein ID: WP_008253910.1
• Coordinates: 1052322..1052510 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN179_RS05245	1048295..1049872	+	1578	WP_014381859.1	serine hydrolase	-
JN179_RS05250	1049869..1052256	+	2388	WP_014711030.1	cation-translocating P-type ATPase	-
JN179_RS05255	1052322..1052510	+	189	WP_008253910.1	antitoxin	Antitoxin
JN179_RS05260	1052514..1052975	+	462	WP_008253911.1	SRPBCC family protein	Toxin
JN179_RS05265	1053060..1053830	+	771	WP_008253913.1	VOC family protein	-
JN179_RS05270	1053904..1054338	+	435	WP_008253914.1	hypothetical protein	-
JN179_RS05275	1054343..1054831	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
JN179_RS05280	1054831..1056357	-	1527	WP_014381862.1	carotenoid oxygenase family protein	-
JN179_RS05285	1056359..1057579	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T38223 WP_008253911.1 NZ_AP024251:1052514-1052975 [Mycobacterium paraintracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T38223 NZ_AP024251:1052514-1052975 [Mycobacterium paraintracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TTGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6797.44 Da
• Isoelectric Point: 4.4702

>AT38223 WP_008253910.1 NZ_AP024251:1052322-1052510 [Mycobacterium paraintracellulare]
MGFLDKAKDLLWQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT38223 NZ_AP024251:1052322-1052510 [Mycobacterium paraintracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTGGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7TPT2