Detailed information of TA system TA038214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2611914..2612578
• Replicon: chromosome
• Accession: NZ_AP024248
• Organism: Mycobacterium intracellulare strain M008

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IUS5
• Locus tag: JN197_RS12375
• Protein ID: WP_014379897.1
• Coordinates: 2612171..2612578 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN197_RS12370
• Protein ID: WP_009954496.1
• Coordinates: 2611914..2612174 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN197_RS12345	2608429..2608920	-	492	WP_095562636.1	DUF1707 domain-containing protein	-
JN197_RS12350	2608990..2609586	+	597	WP_095562637.1	TetR/AcrR family transcriptional regulator	-
JN197_RS12355	2609591..2610859	-	1269	WP_095562638.1	cytochrome P450	-
JN197_RS12360	2611019..2611471	+	453	WP_014379896.1	VOC family protein	-
JN197_RS12365	2611503..2611791	+	289	Protein_2440	hypothetical protein	-
JN197_RS12370	2611914..2612174	+	261	WP_009954496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JN197_RS12375	2612171..2612578	+	408	WP_014379897.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN197_RS12380	2612756..2615857	+	3102	WP_014379898.1	DEAD/DEAH box helicase	-
JN197_RS12385	2615918..2617483	-	1566	WP_014379899.1	serine/threonine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14307.31 Da
• Isoelectric Point: 4.2772

>T38214 WP_014379897.1 NZ_AP024248:2612171-2612578 [Mycobacterium intracellulare]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPIDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T38214 NZ_AP024248:2612171-2612578 [Mycobacterium intracellulare]
ATGAGCAACCCGCAGGTGGCCGGAGTGCTCGACACTTCGGTGTTCATCGCGACCGAAAGTGGTCGGCCTCTCGACGCGGC
GCTGATCCCTGATCAAGTTGGCACGACGGTTGTTACCCTCGCGGAACTGCACGTTGGTGTGTTGGCCGCGTCGACTTCCG
ATATTAGAGCGCAGCGCCTCGCGACATTGGAATCCATTGCGGATATCGAAGCGCTGCCGATCGATGACGATGCGGCCCGG
ATGTGGGCCCGGCTGCGGATCCATCTCGCCGAGACCGGTCAACGCGTCCGGATCAACGATCTGTGGATCGCGGCCATCGC
GGCGTCCCGAGGGCTACCCGTTGTCACGCAGGACGATGACTTTGCCGTCTTGGATGGTGCGGCCAATTTGACCGTTATCC
GCGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT38214 WP_009954496.1 NZ_AP024248:2611914-2612174 [Mycobacterium intracellulare]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT38214 NZ_AP024248:2611914-2612174 [Mycobacterium intracellulare]
ATGTCTGAAGTGGCATCTCGTGAGCTGCGCAACGATACGGCTGGGGTGCTGCGGCGGGTTCGGGATGGAGAGGACGTCAC
CATCACTGTCAGTGGCCGACCGGTCGCAGTTCTTACCGCAGTTGTCCCTCAGCGCAGGCGTTGGCTGAGCAAGACGGAGT
TGCTGTCGCGCTTGCCCCGCGTTCAAGCGGATCCGGGATTACGCGAGGACCTTCACGCACTCGCCGGCGACACTACCGAG
GAGCTGGGCCCGATCCGATGA

Structures

Toxin

• AlphaFold DB: A0A7R7MTD0