Detailed information of TA system TA038197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 889696..890349
• Replicon: chromosome
• Accession: NZ_AP024244
• Organism: Mycobacterium intracellulare strain M005

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN195_RS04545
• Protein ID: WP_008253911.1
• Coordinates: 889888..890349 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: JN195_RS04540
• Protein ID: WP_009957321.1
• Coordinates: 889696..889884 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN195_RS04530	885669..887246	+	1578	WP_009957319.1	serine hydrolase	-
JN195_RS04535	887243..889630	+	2388	WP_009957320.1	cation-translocating P-type ATPase	-
JN195_RS04540	889696..889884	+	189	WP_009957321.1	antitoxin	Antitoxin
JN195_RS04545	889888..890349	+	462	WP_008253911.1	SRPBCC family protein	Toxin
JN195_RS04550	890434..891204	+	771	WP_009957322.1	VOC family protein	-
JN195_RS04555	891278..891712	+	435	WP_008253914.1	hypothetical protein	-
JN195_RS04560	891717..892205	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
JN195_RS04565	892205..893731	-	1527	WP_009957325.1	carotenoid oxygenase family protein	-
JN195_RS04570	893733..894953	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T38197 WP_008253911.1 NZ_AP024244:889888-890349 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T38197 NZ_AP024244:889888-890349 [Mycobacterium intracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TTGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT38197 WP_009957321.1 NZ_AP024244:889696-889884 [Mycobacterium intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT38197 NZ_AP024244:889696-889884 [Mycobacterium intracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTCGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCGGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: X8A187