Detailed information of TA system TA038173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 4246646..4247310
• Replicon: chromosome
• Accession: NZ_AP024237
• Organism: Mycobacterium heckeshornense strain JCM 15655

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MHEC_RS20475
• Protein ID: WP_048892807.1
• Coordinates: 4246646..4247053 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2G8BJ38
• Locus tag: MHEC_RS20480
• Protein ID: WP_048892808.1
• Coordinates: 4247050..4247310 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHEC_RS20465	4244211..4244465	-	255	Protein_4040	IS3 family transposase	-
MHEC_RS20470	4244698..4245324	-	627	WP_048892804.1	hypothetical protein	-
MHEC_RS20475	4246646..4247053	-	408	WP_048892807.1	type II toxin-antitoxin system VapC family toxin	Toxin
MHEC_RS20480	4247050..4247310	-	261	WP_048892808.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MHEC_RS20485	4247760..4250063	-	2304	WP_048892809.1	GH92 family glycosyl hydrolase	-
MHEC_RS20490	4250350..4250556	-	207	WP_200902200.1	DUF86 domain-containing protein	-
MHEC_RS20495	4250584..4250778	-	195	WP_200902201.1	hypothetical protein	-
MHEC_RS20500	4250768..4251151	-	384	WP_048892810.1	nucleotidyltransferase domain-containing protein	-
MHEC_RS20505	4251457..4252221	+	765	WP_048892811.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14340.35 Da
• Isoelectric Point: 4.2062

>T38173 WP_048892807.1 NZ_AP024237:c4247053-4246646 [Mycobacterium heckeshornense]
MSETPPAGLLDTSVFIASETGRQLEESLIPAEVATTVITLAELTVGVLAASTSDSRAQRLATLESVADMETLPVDEEAAR
MWARLRIHLAETGRRVRINDLWIAAIAASRALPVVTQDDDFAALDGAAGLTIIKV

Nucleotide

• Length: 408 bp

>T38173 NZ_AP024237:c4247053-4246646 [Mycobacterium heckeshornense]
ATGAGCGAGACGCCGCCGGCGGGGCTGCTTGACACATCGGTGTTCATCGCCTCCGAAACGGGCCGTCAACTCGAGGAGTC
GCTGATCCCTGCCGAGGTCGCGACTACTGTGATCACTCTTGCCGAACTGACCGTCGGCGTGCTGGCCGCAAGCACTTCCG
ATAGCCGGGCGCAGCGACTCGCGACCCTCGAATCCGTTGCGGATATGGAAACGCTGCCGGTCGATGAGGAAGCGGCGCGC
ATGTGGGCGCGGCTACGCATCCACCTGGCCGAGACTGGCCGTCGAGTCCGTATCAACGACCTGTGGATCGCAGCCATCGC
TGCTTCGCGAGCGCTGCCCGTCGTCACCCAGGACGACGACTTTGCCGCCCTGGACGGCGCGGCCGGTCTCACGATCATCA
AAGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9323.63 Da
• Isoelectric Point: 11.1777

>AT38173 WP_048892808.1 NZ_AP024237:c4247310-4247050 [Mycobacterium heckeshornense]
MSDVASRDLRNDTAGVLRRVQAGEDITITVKGRPVAMLTAAGPPRRRWLAKTEFLARLRRAQADPGLRDDLGLLAGDTTD
DLGPAR

Nucleotide

• Length: 261 bp

>AT38173 NZ_AP024237:c4247310-4247050 [Mycobacterium heckeshornense]
ATGTCCGATGTGGCTTCCCGTGACTTGCGCAACGATACGGCGGGAGTGCTTCGCCGCGTCCAGGCCGGAGAAGACATCAC
CATCACGGTCAAAGGCCGCCCCGTCGCAATGCTCACCGCGGCCGGGCCTCCGCGTCGGCGCTGGTTGGCCAAAACCGAGT
TTCTGGCACGGTTGCGCCGTGCGCAAGCCGATCCAGGTTTGCGCGACGACCTTGGGTTGCTCGCCGGCGACACCACCGAC
GATCTCGGTCCTGCCCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A2G8BJ38