Detailed information of TA system TA038171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4177364..4177986
• Replicon: chromosome
• Accession: NZ_AP024237
• Organism: Mycobacterium heckeshornense strain JCM 15655

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MHEC_RS20185
• Protein ID: WP_048890327.1
• Coordinates: 4177364..4177762 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2G8BJ40
• Locus tag: MHEC_RS20190
• Protein ID: WP_048890328.1
• Coordinates: 4177759..4177986 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHEC_RS20160	4172517..4174235	+	1719	WP_048890320.1	exodeoxyribonuclease V subunit alpha	-
MHEC_RS20165	4174342..4174695	-	354	WP_048890321.1	VOC family protein	-
MHEC_RS20170	4175348..4175599	+	252	WP_082169627.1	YdeI/OmpD-associated family protein	-
MHEC_RS20175	4175612..4176559	-	948	WP_048890325.1	class I SAM-dependent methyltransferase	-
MHEC_RS20180	4176593..4177324	+	732	WP_048890326.1	TVP38/TMEM64 family protein	-
MHEC_RS20185	4177364..4177762	-	399	WP_048890327.1	PIN domain-containing protein	Toxin
MHEC_RS20190	4177759..4177986	-	228	WP_048890328.1	hypothetical protein	Antitoxin
MHEC_RS20195	4178105..4180663	+	2559	Protein_3986	SEC-C domain-containing protein	-
MHEC_RS20200	4180702..4181856	+	1155	WP_048890329.1	FIST C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 13934.64 Da
• Isoelectric Point: 6.6927

>T38171 WP_048890327.1 NZ_AP024237:c4177762-4177364 [Mycobacterium heckeshornense]
VTALLDANVLIALVVSEHVHHDAAAEWLSASGTAFATCPITQGSLVRFLLRSGQSAAAARDVVAGVENAHRHEFWSDSVS
FADVAFSGVVGHRQVTDAYLAQLARSRNGRLATLDSALAHQHSDVAVLIPAR

Nucleotide

• Length: 399 bp

>T38171 NZ_AP024237:c4177762-4177364 [Mycobacterium heckeshornense]
GTGACGGCGTTGCTCGACGCGAATGTGCTCATCGCGTTGGTTGTCAGCGAGCACGTGCATCATGATGCCGCCGCCGAGTG
GCTTTCGGCATCCGGCACGGCGTTCGCGACGTGCCCGATCACGCAGGGAAGTCTTGTCCGGTTTCTGTTGCGGTCTGGGC
AATCCGCGGCAGCCGCGCGGGATGTCGTGGCCGGGGTCGAAAATGCGCATCGTCACGAGTTTTGGTCTGACAGTGTCTCT
TTCGCCGATGTGGCGTTCAGCGGTGTGGTCGGGCACCGGCAGGTAACCGATGCCTATCTTGCCCAGCTGGCCCGAAGCCG
CAACGGCCGGCTGGCGACTCTGGATAGCGCCCTGGCGCACCAGCACAGCGATGTCGCGGTGCTTATCCCTGCGCGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8021.98 Da
• Isoelectric Point: 4.6842

>AT38171 WP_048890328.1 NZ_AP024237:c4177986-4177759 [Mycobacterium heckeshornense]
VRTTIDLPDDLHKQALAIARDTHRTLSETVADLIRRGLSTGSVAAISTDPRTGLPLVSVGTVVTSEDVRALEDEQ

Nucleotide

• Length: 228 bp

>AT38171 NZ_AP024237:c4177986-4177759 [Mycobacterium heckeshornense]
GTGCGTACCACCATCGATCTTCCCGATGATCTGCACAAACAGGCGCTGGCGATCGCCCGCGATACCCACCGCACGTTGAG
CGAAACGGTCGCCGACCTCATCCGGCGTGGCCTGAGCACGGGCAGCGTCGCGGCGATCTCCACGGATCCCAGAACCGGGT
TGCCTCTGGTGAGCGTCGGAACGGTCGTGACTTCAGAAGATGTGCGCGCATTGGAGGATGAGCAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2G8BJ40