Detailed information of TA system TA038168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1826553..1827134
• Replicon: chromosome
• Accession: NZ_AP024233
• Organism: Desulfolithobacter dissulfuricans strain GF1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GF1_RS08105
• Protein ID: WP_267926025.1
• Coordinates: 1826835..1827134 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GF1_RS08100
• Protein ID: WP_267926024.1
• Coordinates: 1826553..1826831 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GF1_RS08070 (GF1_16370)	1821843..1822022	+	180	WP_267926017.1	hypothetical protein	-
GF1_RS08075 (GF1_16380)	1822233..1823624	+	1392	WP_267926018.1	transposase	-
GF1_RS08080 (GF1_16390)	1823863..1824336	+	474	WP_267926020.1	hypothetical protein	-
GF1_RS08085 (GF1_16400)	1824323..1825378	+	1056	WP_267926021.1	TraU family protein	-
GF1_RS08090 (GF1_16410)	1825378..1826178	+	801	WP_267926022.1	type-F conjugative transfer system pilin assembly protein TrbC	-
GF1_RS08095 (GF1_16420)	1826196..1826456	+	261	WP_267926023.1	hypothetical protein	-
GF1_RS08100 (GF1_16430)	1826553..1826831	-	279	WP_267926024.1	putative addiction module antidote protein	Antitoxin
GF1_RS08105 (GF1_16440)	1826835..1827134	-	300	WP_267926025.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GF1_RS08110 (GF1_16450)	1827184..1827528	+	345	WP_267926026.1	hypothetical protein	-
GF1_RS08115 (GF1_16460)	1827491..1827976	+	486	WP_267926027.1	S26 family signal peptidase	-
GF1_RS08120 (GF1_16470)	1827927..1828838	+	912	WP_267926028.1	conjugal transfer protein TraF	-
GF1_RS08125 (GF1_16480)	1828817..1829536	+	720	WP_267926029.1	hypothetical protein	-
GF1_RS08130 (GF1_16490)	1829617..1831089	+	1473	WP_267926030.1	conjugal transfer protein TraH	-
GF1_RS08135 (GF1_16500)	1831325..1832134	-	810	WP_267926031.1	CsgG/HfaB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1781020..1916375	135355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11524.21 Da
• Isoelectric Point: 9.6757

>T38168 WP_267926025.1 NZ_AP024233:c1827134-1826835 [Desulfolithobacter dissulfuricans]
MEYTLRSTKEYDKWFARLKDSQAKIRILARLSRVENGNFGDYKPLGPNLFELRFFFGPGWRIYYTIKNNSVVLLLVGGEK
SSQEKNIAKATELLSELED

Nucleotide

• Length: 300 bp

>T38168 NZ_AP024233:c1827134-1826835 [Desulfolithobacter dissulfuricans]
ATGGAATACACGTTGCGCAGCACAAAAGAATACGACAAGTGGTTTGCCAGGTTGAAGGACTCCCAAGCCAAAATCAGGAT
ACTGGCCAGGCTCAGCCGAGTTGAAAACGGCAATTTTGGAGACTACAAGCCGCTTGGTCCCAATCTGTTTGAGCTGCGTT
TCTTTTTTGGCCCAGGCTGGCGCATTTATTACACCATCAAGAACAATAGCGTTGTTTTGTTGTTGGTTGGTGGCGAAAAA
TCGAGCCAGGAAAAAAACATTGCCAAAGCTACAGAGCTTTTATCCGAACTGGAGGATTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9907.53 Da
• Isoelectric Point: 9.7179

>AT38168 WP_267926024.1 NZ_AP024233:c1826831-1826553 [Desulfolithobacter dissulfuricans]
MALKTKPFDIAEHLKTAEDIRLFLQEVAATGDEADFIHALSIAARAKGMTEIAKKAGVTRASLYKSLSEDGNPRFTTISK
ITKALGCKLSIA

Nucleotide

• Length: 279 bp

>AT38168 NZ_AP024233:c1826831-1826553 [Desulfolithobacter dissulfuricans]
ATGGCCCTTAAAACAAAACCTTTTGACATTGCCGAACATTTGAAGACTGCTGAGGATATTCGCCTTTTCTTGCAGGAGGT
AGCTGCTACCGGAGACGAAGCCGACTTTATCCATGCCCTGAGTATTGCAGCAAGGGCGAAAGGGATGACCGAAATAGCCA
AAAAAGCAGGGGTCACCAGAGCCAGTCTGTACAAATCTCTATCCGAAGATGGCAATCCAAGATTCACTACCATCAGCAAG
ATCACAAAAGCGCTTGGCTGCAAACTATCCATTGCTTGA