Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4270640..4271137 Replicon chromosome
Accession NZ_AP024205
Organism Escherichia coli strain TA8571

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JG732_RS20735 Protein ID WP_001173343.1
Coordinates 4270640..4270813 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JG732_RS20740 Protein ID WP_000398619.1
Coordinates 4270841..4271137 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG732_RS20725 4267310..4268767 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
JG732_RS20730 4269004..4270521 + 1518 WP_063084812.1 lysine--tRNA ligase -
JG732_RS20735 4270640..4270813 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JG732_RS20740 4270841..4271137 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JG732_RS20745 4271364..4271636 - 273 WP_000405651.1 N-acetyltransferase -
JG732_RS20750 4271648..4271878 - 231 WP_063084813.1 (4Fe-4S)-binding protein -
JG732_RS20755 4272059..4273690 + 1632 WP_201409613.1 sensor histidine kinase -
JG732_RS20760 4273687..4274406 + 720 WP_000611288.1 two-component system response regulator DcuR -
JG732_RS20765 4274455..4275152 - 698 Protein_4067 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4274455..4274748 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T38167 WP_001173343.1 NZ_AP024205:c4270813-4270640 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T38167 NZ_AP024205:c4270813-4270640 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT38167 WP_000398619.1 NZ_AP024205:c4271137-4270841 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT38167 NZ_AP024205:c4271137-4270841 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References