Detailed information of TA system TA038135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 75386..75915
• Replicon: chromosome
• Accession: NZ_AP024203
• Organism: Staphylococcus aureus strain SA23-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SA231_RS00470
• Protein ID: WP_000621175.1
• Coordinates: 75386..75748 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SA231_RS00475
• Protein ID: WP_000948331.1
• Coordinates: 75745..75915 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SA231_RS00450 (72365)	72365..73135	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SA231_RS00455 (73110)	73110..73589	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SA231_RS00460 (73591)	73591..73917	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SA231_RS00465 (74036)	74036..75037	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SA231_RS00470 (75386)	75386..75748	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SA231_RS00475 (75745)	75745..75915	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SA231_RS00480 (76000)	76000..77148	-	1149	WP_117220098.1	alanine racemase	-
SA231_RS00485 (77214)	77214..77573	-	360	WP_000581199.1	holo-ACP synthase	-
SA231_RS00490 (77577)	77577..78068	-	492	WP_001205907.1	PH domain-containing protein	-
SA231_RS00495 (78055)	78055..79638	-	1584	WP_229345018.1	PH domain-containing protein	-
SA231_RS00500 (79631)	79631..80110	-	480	WP_001287077.1	hypothetical protein	-
SA231_RS00505 (80318)	80318..80878	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T38135 WP_000621175.1 NZ_AP024203:c75748-75386 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T38135 NZ_AP024203:c75748-75386 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT38135 WP_000948331.1 NZ_AP024203:c75915-75745 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT38135 NZ_AP024203:c75915-75745 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1