Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 72677..73413 Replicon plasmid pKSB1_9A_1
Accession NZ_AP024174
Organism Klebsiella pneumoniae strain KSB1_9A

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag JGR95_RS26040 Protein ID WP_003026803.1
Coordinates 72677..73159 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag JGR95_RS26045 Protein ID WP_003026799.1
Coordinates 73147..73413 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGR95_RS26020 68129..68761 + 633 WP_001567369.1 hypothetical protein -
JGR95_RS26025 68790..70193 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
JGR95_RS26030 70412..71044 + 633 WP_001567369.1 hypothetical protein -
JGR95_RS26035 71073..72476 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
JGR95_RS26040 72677..73159 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
JGR95_RS26045 73147..73413 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
JGR95_RS26050 73858..74088 - 231 WP_004206660.1 hypothetical protein -
JGR95_RS26055 74102..74305 - 204 WP_004206661.1 hemolysin expression modulator Hha -
JGR95_RS26060 74366..74857 - 492 WP_004206662.1 hypothetical protein -
JGR95_RS26065 74991..76202 - 1212 WP_032415728.1 hypothetical protein -
JGR95_RS26070 76199..76447 - 249 WP_004193994.1 hypothetical protein -
JGR95_RS26075 76805..77155 + 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
JGR95_RS26080 77207..77569 + 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..252273 252273


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T38105 WP_003026803.1 NZ_AP024174:c73159-72677 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T38105 NZ_AP024174:c73159-72677 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT38105 WP_003026799.1 NZ_AP024174:c73413-73147 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT38105 NZ_AP024174:c73413-73147 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References