Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18212..18855 Replicon plasmid pKSB1_9A_1
Accession NZ_AP024174
Organism Klebsiella pneumoniae strain KSB1_9A

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag JGR95_RS25790 Protein ID WP_032425563.1
Coordinates 18439..18855 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag JGR95_RS25785 Protein ID WP_001261276.1
Coordinates 18212..18442 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGR95_RS25750 13991..14248 + 258 WP_009310077.1 hypothetical protein -
JGR95_RS25755 14306..15085 - 780 WP_023287113.1 site-specific integrase -
JGR95_RS25760 15283..16299 - 1017 WP_009309921.1 hypothetical protein -
JGR95_RS25765 16333..16668 - 336 WP_009309920.1 hypothetical protein -
JGR95_RS25770 16718..17029 - 312 WP_023287114.1 hypothetical protein -
JGR95_RS25775 17087..17392 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -
JGR95_RS25780 17394..17612 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
JGR95_RS25785 18212..18442 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JGR95_RS25790 18439..18855 + 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
JGR95_RS25795 18896..19774 - 879 WP_009309916.1 restriction endonuclease -
JGR95_RS25800 20436..21497 + 1062 Protein_24 HlyD family secretion protein -
JGR95_RS25805 21646..22626 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
JGR95_RS25810 22816..23724 + 909 WP_032425603.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..252273 252273
- flank IS/Tn - - 21646..22626 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T38104 WP_032425563.1 NZ_AP024174:18439-18855 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T38104 NZ_AP024174:18439-18855 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT38104 WP_001261276.1 NZ_AP024174:18212-18442 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT38104 NZ_AP024174:18212-18442 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References