Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 17087..17612 Replicon plasmid pKSB1_9A_1
Accession NZ_AP024174
Organism Klebsiella pneumoniae strain KSB1_9A

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag JGR95_RS25775 Protein ID WP_004197633.1
Coordinates 17087..17392 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag JGR95_RS25780 Protein ID WP_006788213.1
Coordinates 17394..17612 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JGR95_RS25745 12884..13864 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
JGR95_RS25750 13991..14248 + 258 WP_009310077.1 hypothetical protein -
JGR95_RS25755 14306..15085 - 780 WP_023287113.1 site-specific integrase -
JGR95_RS25760 15283..16299 - 1017 WP_009309921.1 hypothetical protein -
JGR95_RS25765 16333..16668 - 336 WP_009309920.1 hypothetical protein -
JGR95_RS25770 16718..17029 - 312 WP_023287114.1 hypothetical protein -
JGR95_RS25775 17087..17392 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
JGR95_RS25780 17394..17612 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JGR95_RS25785 18212..18442 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JGR95_RS25790 18439..18855 + 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin -
JGR95_RS25795 18896..19774 - 879 WP_009309916.1 restriction endonuclease -
JGR95_RS25800 20436..21497 + 1062 Protein_24 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..252273 252273
- flank IS/Tn - - 21646..22626 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T38103 WP_004197633.1 NZ_AP024174:c17392-17087 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T38103 NZ_AP024174:c17392-17087 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT38103 WP_006788213.1 NZ_AP024174:c17612-17394 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT38103 NZ_AP024174:c17612-17394 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References