Detailed information of TA system TA038098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3963585..3964204
• Replicon: chromosome
• Accession: NZ_AP024173
• Organism: Klebsiella pneumoniae strain KSB1_9A

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: JGR95_RS19610
• Protein ID: WP_002892050.1
• Coordinates: 3963986..3964204 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: JGR95_RS19605
• Protein ID: WP_201357807.1
• Coordinates: 3963585..3963959 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGR95_RS19595	3958737..3959930	+	1194	WP_004177236.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
JGR95_RS19600	3959953..3963099	+	3147	WP_102024722.1	multidrug efflux RND transporter permease subunit AcrB	-
JGR95_RS19605	3963585..3963959	+	375	WP_201357807.1	Hha toxicity modulator TomB	Antitoxin
JGR95_RS19610	3963986..3964204	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
JGR95_RS19615	3964363..3964929	+	567	WP_002892030.1	maltose O-acetyltransferase	-
JGR95_RS19620	3964901..3965041	-	141	WP_004147370.1	hypothetical protein	-
JGR95_RS19625	3965062..3965532	+	471	WP_002892026.1	YlaC family protein	-
JGR95_RS19630	3965507..3966958	-	1452	WP_064167449.1	PLP-dependent aminotransferase family protein	-
JGR95_RS19635	3967059..3967757	+	699	WP_135664576.1	GNAT family N-acetyltransferase	-
JGR95_RS19640	3967754..3967894	-	141	WP_002892018.1	type B 50S ribosomal protein L36	-
JGR95_RS19645	3967894..3968157	-	264	WP_002892011.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T38098 WP_002892050.1 NZ_AP024173:3963986-3964204 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T38098 NZ_AP024173:3963986-3964204 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14402.08 Da
• Isoelectric Point: 4.8989

>AT38098 WP_201357807.1 NZ_AP024173:3963585-3963959 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELFEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT38098 NZ_AP024173:3963585-3963959 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGTTCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2