Detailed information of TA system TA038060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 82324..82934
• Replicon: plasmid pM311693
• Accession: NZ_AP024162
• Organism: Ralstonia solanacearum strain MAFF 311693

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0K1ZQG6
• Locus tag: JL096_RS17715
• Protein ID: WP_028853939.1
• Coordinates: 82626..82934 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JL096_RS17710
• Protein ID: WP_016722339.1
• Coordinates: 82324..82629 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL096_RS17685	78642..78923	-	282	WP_019719760.1	hypothetical protein	-
JL096_RS17690	79054..79338	-	285	WP_011003391.1	hypothetical protein	-
JL096_RS17695	79643..79860	+	218	Protein_70	hypothetical protein	-
JL096_RS17700	80027..80692	+	666	WP_197368577.1	hypothetical protein	-
JL096_RS17705	80798..82111	-	1314	WP_071507363.1	metabolite/H+ symporter	-
JL096_RS17710	82324..82629	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
JL096_RS17715	82626..82934	-	309	WP_028853939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL096_RS17720	83010..84177	-	1168	Protein_75	MFS transporter	-
JL096_RS17725	84304..85125	-	822	WP_011003398.1	GntR family transcriptional regulator	-
JL096_RS17730	85303..85854	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
JL096_RS17735	85987..86586	+	600	WP_011003400.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / adeG / acrB / fliI / fliG / fliM / flgG / flgC / cheY / cheB / cheW / motA	1..2117975	2117975

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11841.61 Da
• Isoelectric Point: 7.1367

>T38060 WP_028853939.1 NZ_AP024162:c82934-82626 [Ralstonia solanacearum]
MLTIRTTEIFDDWFCSLRDKAAQRRIQVRIDRLQMGNPGDMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T38060 NZ_AP024162:c82934-82626 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAAATCTTCGACGACTGGTTTTGCAGCCTCCGCGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGGCAACCCGGGCGACATGAAAGCGGTCCGTGACGGCATCCGTGAACTGCGGATCG
ATCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT38060 WP_016722339.1 NZ_AP024162:c82629-82324 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT38060 NZ_AP024162:c82629-82324 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACCGGGATGACCCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTCGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Toxin

• AlphaFold DB: A0A0K1ZQG6