Detailed information of TA system TA038058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 492695..493328
• Replicon: chromosome
• Accession: NZ_AP024161
• Organism: Ralstonia solanacearum strain MAFF 311693

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JL096_RS02240
• Protein ID: WP_197369056.1
• Coordinates: 492915..493328 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8XUX7
• Locus tag: JL096_RS02235
• Protein ID: WP_011002949.1
• Coordinates: 492695..492928 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL096_RS02220	488283..489578	-	1296	WP_011002952.1	MFS transporter	-
JL096_RS02225	489586..490836	-	1251	WP_197369055.1	AGE family epimerase/isomerase	-
JL096_RS02230	491031..492638	+	1608	WP_020832850.1	glucan biosynthesis protein	-
JL096_RS02235	492695..492928	+	234	WP_011002949.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JL096_RS02240	492915..493328	+	414	WP_197369056.1	PIN domain-containing protein	Toxin
JL096_RS02245	493303..493929	-	627	WP_016725173.1	DUF1415 domain-containing protein	-
JL096_RS02250	494120..494491	+	372	WP_165591128.1	hypothetical protein	-
JL096_RS02255	494519..495739	-	1221	WP_011002488.1	ISL3-like element ISRso15 family transposase	-
JL096_RS02260	495870..497366	-	1497	WP_011002945.1	glycerol kinase GlpK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	494519..495739	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15117.51 Da
• Isoelectric Point: 5.5348

>T38058 WP_197369056.1 NZ_AP024161:492915-493328 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIEMVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Nucleotide

• Length: 414 bp

>T38058 NZ_AP024161:492915-493328 [Ralstonia solanacearum]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGGC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTGATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAATGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8790.98 Da
• Isoelectric Point: 8.7287

>AT38058 WP_011002949.1 NZ_AP024161:492695-492928 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Nucleotide

• Length: 234 bp

>AT38058 NZ_AP024161:492695-492928 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTCCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A223GR76