Detailed information of TA system TA038049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 458176..458809
• Replicon: chromosome
• Accession: NZ_AP024157
• Organism: Ralstonia solanacearum strain MAFF 211472

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JL095_RS02185
• Protein ID: WP_021155142.1
• Coordinates: 458396..458809 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8XUX7
• Locus tag: JL095_RS02180
• Protein ID: WP_011002949.1
• Coordinates: 458176..458409 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL095_RS02165	453764..455059	-	1296	WP_011002952.1	MFS transporter	-
JL095_RS02170	455067..456317	-	1251	WP_096761512.1	AGE family epimerase/isomerase	-
JL095_RS02175	456512..458119	+	1608	WP_020832850.1	glucan biosynthesis protein	-
JL095_RS02180	458176..458409	+	234	WP_011002949.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JL095_RS02185	458396..458809	+	414	WP_021155142.1	PIN domain-containing protein	Toxin
JL095_RS02190	458784..459410	-	627	WP_016725173.1	DUF1415 domain-containing protein	-
JL095_RS02195	459601..459972	+	372	WP_016725174.1	hypothetical protein	-
JL095_RS02200	460037..461533	-	1497	WP_016725175.1	glycerol kinase GlpK	-
JL095_RS02205	461695..463431	-	1737	WP_016725176.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15117.45 Da
• Isoelectric Point: 5.5348

>T38049 WP_021155142.1 NZ_AP024157:458396-458809 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADATETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Nucleotide

• Length: 414 bp

>T38049 NZ_AP024157:458396-458809 [Ralstonia solanacearum]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGAC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTGATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAACGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8790.98 Da
• Isoelectric Point: 8.7287

>AT38049 WP_011002949.1 NZ_AP024157:458176-458409 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Nucleotide

• Length: 234 bp

>AT38049 NZ_AP024157:458176-458409 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTCCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A223GR76