Detailed information of TA system TA038048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 496..959
• Replicon: plasmid pMYT19855
• Accession: NZ_AP024156
• Organism: Neisseria gonorrhoeae strain TUM 19855

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: Q51003
• Locus tag: JLZ01_RS11365
• Protein ID: WP_003690225.1
• Coordinates: 681..959 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: Q51002
• Locus tag: JLZ01_RS11360
• Protein ID: WP_003690223.1
• Coordinates: 496..681 (+)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLZ01_RS11350	9..251	+	243	WP_003690220.1	HTH domain-containing protein	-
JLZ01_RS11355	248..493	+	246	WP_003690221.1	hypothetical protein	-
JLZ01_RS11360	496..681	+	186	WP_003690223.1	DUF5397 family protein	Antitoxin
JLZ01_RS11365	681..959	+	279	WP_003690225.1	endoribonuclease VapD	Toxin
JLZ01_RS11370	937..1206	-	270	WP_003692166.1	hypothetical protein	-
JLZ01_RS11375	1238..1825	-	588	WP_003692168.1	B protein	-
JLZ01_RS11380	1909..2538	-	630	WP_003696452.1	relaxase/mobilization nuclease domain-containing protein	-
JLZ01_RS11385	2523..2714	-	192	WP_003690218.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4153	4153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (2-60)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 6.9726

>T38048 WP_003690225.1 NZ_AP024156:681-959 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Nucleotide

• Length: 279 bp

>T38048 NZ_AP024156:681-959 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 6717.54 Da
• Isoelectric Point: 4.2641

>AT38048 WP_003690223.1 NZ_AP024156:496-681 [Neisseria gonorrhoeae]
MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESGEHTQYKLSHIVQDPKAA

Nucleotide

• Length: 186 bp

>AT38048 NZ_AP024156:496-681 [Neisseria gonorrhoeae]
ATGCAAACCACTATCCCCACCGGTTCAATCCGCACCTTTGGCGATTACGGCGTGATGTACATCGTCGGCACGCCTGCCGA
ACAGCTTCCGGACGGCGATTGGCTCGTAAATATTGAACTGCCGGAAAGCGGCGAACACACGCAATACAAACTGTCCCACA
TCGTTCAAGACCCAAAGGCTGCCTAA

Structures

Toxin

• AlphaFold DB: A0A7H5FJI5

Antitoxin

• AlphaFold DB: Q51002