Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4596723..4597220 Replicon chromosome
Accession NZ_AP024130
Organism Escherichia coli strain SI-NP020

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FFK26_RS22055 Protein ID WP_001173343.1
Coordinates 4596723..4596896 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FFK26_RS22060 Protein ID WP_000398619.1
Coordinates 4596924..4597220 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFK26_RS22045 (4593393) 4593393..4594850 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
FFK26_RS22050 (4595087) 4595087..4596604 + 1518 WP_001295074.1 lysine--tRNA ligase -
FFK26_RS22055 (4596723) 4596723..4596896 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FFK26_RS22060 (4596924) 4596924..4597220 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FFK26_RS22065 (4597447) 4597447..4597719 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
FFK26_RS22070 (4597731) 4597731..4597961 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
FFK26_RS22075 (4598142) 4598142..4599773 + 1632 WP_001216486.1 sensor histidine kinase -
FFK26_RS22080 (4599770) 4599770..4600489 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4586552..4597220 10668


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T38038 WP_001173343.1 NZ_AP024130:c4596896-4596723 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T38038 NZ_AP024130:c4596896-4596723 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT38038 WP_000398619.1 NZ_AP024130:c4597220-4596924 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT38038 NZ_AP024130:c4597220-4596924 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTAAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References