Detailed information of TA system TA038033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3705522..3706359
• Replicon: chromosome
• Accession: NZ_AP024130
• Organism: Escherichia coli strain SI-NP020

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: FFK26_RS17990
• Protein ID: WP_000227784.1
• Coordinates: 3705817..3706359 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: FFK26_RS17985
• Protein ID: WP_001297137.1
• Coordinates: 3705522..3705833 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFK26_RS17960 (3700542)	3700542..3701489	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
FFK26_RS17965 (3701511)	3701511..3703502	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FFK26_RS17970 (3703492)	3703492..3704106	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FFK26_RS17975 (3704106)	3704106..3704435	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
FFK26_RS17980 (3704447)	3704447..3705337	+	891	WP_000971336.1	heme o synthase	-
FFK26_RS17985 (3705522)	3705522..3705833	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
FFK26_RS17990 (3705817)	3705817..3706359	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
FFK26_RS17995 (3706415)	3706415..3707350	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
FFK26_RS18000 (3707758)	3707758..3709122	+	1365	WP_001000978.1	MFS transporter	-
FFK26_RS18005 (3709250)	3709250..3709741	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
FFK26_RS18010 (3709909)	3709909..3710820	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T38033 WP_000227784.1 NZ_AP024130:3705817-3706359 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T38033 NZ_AP024130:3705817-3706359 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT38033 WP_001297137.1 NZ_AP024130:3705522-3705833 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT38033 NZ_AP024130:3705522-3705833 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3