Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1050008..1050591 Replicon chromosome
Accession NZ_AP024130
Organism Escherichia coli strain SI-NP020

Toxin (Protein)


Gene name mazF Uniprot ID F4NJF2
Locus tag FFK26_RS05120 Protein ID WP_000254731.1
Coordinates 1050256..1050591 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag FFK26_RS05115 Protein ID WP_000581937.1
Coordinates 1050008..1050256 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFK26_RS05105 (1046347) 1046347..1047648 + 1302 WP_000046800.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
FFK26_RS05110 (1047696) 1047696..1049930 + 2235 WP_137564240.1 GTP diphosphokinase -
FFK26_RS05115 (1050008) 1050008..1050256 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FFK26_RS05120 (1050256) 1050256..1050591 + 336 WP_000254731.1 endoribonuclease MazF Toxin
FFK26_RS05125 (1050662) 1050662..1051453 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
FFK26_RS05130 (1051681) 1051681..1053318 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
FFK26_RS05135 (1053406) 1053406..1054704 + 1299 WP_000036723.1 phosphopyruvate hydratase -
FFK26_RS05140 (1054688) 1054688..1054828 - 141 WP_000718545.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12043.02 Da        Isoelectric Point: 8.1170

>T38017 WP_000254731.1 NZ_AP024130:1050256-1050591 [Escherichia coli]
MVSRYVPDMGDLICVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Download         Length: 336 bp

>T38017 NZ_AP024130:1050256-1050591 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGTGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGTCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT38017 WP_000581937.1 NZ_AP024130:1050008-1050256 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT38017 NZ_AP024130:1050008-1050256 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F4NJF2

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References