Detailed information of TA system TA038008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 15991..16616
• Replicon: plasmid NS-NP030_P1
• Accession: NZ_AP024127
• Organism: Escherichia coli strain NS-NP030

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FFJ14_RS25755
• Protein ID: WP_000911317.1
• Coordinates: 15991..16389 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q31SG2
• Locus tag: FFJ14_RS25760
• Protein ID: WP_000450530.1
• Coordinates: 16389..16616 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFJ14_RS25735 (11578)	11578..12075	+	498	WP_063113258.1	entry exclusion protein	-
FFJ14_RS25740 (12107)	12107..12838	+	732	WP_000850424.1	conjugal transfer complement resistance protein TraT	-
FFJ14_RS25745 (13041)	13041..13778	+	738	WP_063113257.1	hypothetical protein	-
FFJ14_RS25750 (13829)	13829..15982	+	2154	WP_063113256.1	type IV conjugative transfer system coupling protein TraD	-
FFJ14_RS25755 (15991)	15991..16389	-	399	WP_000911317.1	type II toxin-antitoxin system VapC family toxin	Toxin
FFJ14_RS25760 (16389)	16389..16616	-	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / cnf1	1..127587	127587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T38008 WP_000911317.1 NZ_AP024127:c16389-15991 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T38008 NZ_AP024127:c16389-15991 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAATATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.59 Da
• Isoelectric Point: 4.7513

>AT38008 WP_000450530.1 NZ_AP024127:c16616-16389 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT38008 NZ_AP024127:c16616-16389 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: Q31SG2