Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4153183..4153837 Replicon chromosome
Accession NZ_AP024126
Organism Escherichia coli strain NS-NP030

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag FFJ14_RS20185 Protein ID WP_000244772.1
Coordinates 4153183..4153590 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FFJ14_RS20190 Protein ID WP_000354046.1
Coordinates 4153571..4153837 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFJ14_RS20165 (4149140) 4149140..4150873 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
FFJ14_RS20170 (4150879) 4150879..4151589 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FFJ14_RS20175 (4151614) 4151614..4152510 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FFJ14_RS20180 (4152622) 4152622..4153143 + 522 WP_001055874.1 flavodoxin FldB -
FFJ14_RS20185 (4153183) 4153183..4153590 - 408 WP_000244772.1 protein YgfX Toxin
FFJ14_RS20190 (4153571) 4153571..4153837 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FFJ14_RS20195 (4154080) 4154080..4155060 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FFJ14_RS20200 (4155313) 4155313..4156293 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
FFJ14_RS20205 (4156416) 4156416..4157075 - 660 WP_000250274.1 hemolysin III family protein -
FFJ14_RS20210 (4157239) 4157239..4157550 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4155313..4156293 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T38002 WP_000244772.1 NZ_AP024126:c4153590-4153183 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T38002 NZ_AP024126:c4153590-4153183 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT38002 WP_000354046.1 NZ_AP024126:c4153837-4153571 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT38002 NZ_AP024126:c4153837-4153571 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References