Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4016916..4017499 Replicon chromosome
Accession NZ_AP024126
Organism Escherichia coli strain NS-NP030

Toxin (Protein)


Gene name mazF Uniprot ID A0A8S7BDL1
Locus tag FFJ14_RS19560 Protein ID WP_063113611.1
Coordinates 4016916..4017251 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag FFJ14_RS19565 Protein ID WP_000581937.1
Coordinates 4017251..4017499 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFJ14_RS19545 (4012803) 4012803..4014101 - 1299 WP_000036723.1 phosphopyruvate hydratase -
FFJ14_RS19550 (4014189) 4014189..4015826 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
FFJ14_RS19555 (4016054) 4016054..4016845 - 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
FFJ14_RS19560 (4016916) 4016916..4017251 - 336 WP_063113611.1 endoribonuclease MazF Toxin
FFJ14_RS19565 (4017251) 4017251..4017499 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FFJ14_RS19570 (4017577) 4017577..4019811 - 2235 WP_000226815.1 GTP pyrophosphokinase -
FFJ14_RS19575 (4019859) 4019859..4021160 - 1302 WP_000046800.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12097.06 Da        Isoelectric Point: 8.7218

>T38001 WP_063113611.1 NZ_AP024126:c4017251-4016916 [Escherichia coli]
MVSRYVPDMGDLIWVDFNPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T38001 NZ_AP024126:c4017251-4016916 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTAACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT38001 WP_000581937.1 NZ_AP024126:c4017499-4017251 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT38001 NZ_AP024126:c4017499-4017251 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References