Detailed information of TA system TA038000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3709118..3709743
• Replicon: chromosome
• Accession: NZ_AP024126
• Organism: Escherichia coli strain NS-NP030

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FFJ14_RS18060
• Protein ID: WP_000911330.1
• Coordinates: 3709118..3709516 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FFJ14_RS18065
• Protein ID: WP_000450524.1
• Coordinates: 3709516..3709743 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFJ14_RS18040 (3705025)	3705025..3705225	+	201	WP_000383836.1	YpfN family protein	-
FFJ14_RS18045 (3705306)	3705306..3706004	-	699	WP_000679812.1	esterase	-
FFJ14_RS18050 (3706078)	3706078..3708093	-	2016	WP_033885019.1	tRNA cytosine(34) acetyltransferase TmcA	-
FFJ14_RS18055 (3708108)	3708108..3708971	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
FFJ14_RS18060 (3709118)	3709118..3709516	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
FFJ14_RS18065 (3709516)	3709516..3709743	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FFJ14_RS18070 (3709897)	3709897..3710610	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FFJ14_RS18075 (3710823)	3710823..3711857	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FFJ14_RS18080 (3711874)	3711874..3712752	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FFJ14_RS18085 (3712898)	3712898..3713470	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FFJ14_RS18090 (3713470)	3713470..3713940	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T38000 WP_000911330.1 NZ_AP024126:c3709516-3709118 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T38000 NZ_AP024126:c3709516-3709118 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT38000 WP_000450524.1 NZ_AP024126:c3709743-3709516 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT38000 NZ_AP024126:c3709743-3709516 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33