Detailed information of TA system TA037988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2371721..2372359
• Replicon: chromosome
• Accession: NZ_AP024126
• Organism: Escherichia coli strain NS-NP030

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FFJ14_RS11585
• Protein ID: WP_000813794.1
• Coordinates: 2371721..2371897 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FFJ14_RS11590
• Protein ID: WP_001270286.1
• Coordinates: 2371943..2372359 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFJ14_RS11565 (2367340)	2367340..2368515	-	1176	WP_063103682.1	BenE family transporter YdcO	-
FFJ14_RS11570 (2368607)	2368607..2369143	+	537	WP_063103683.1	DNA-binding transcriptional regulator SutR	-
FFJ14_RS11575 (2369216)	2369216..2371177	+	1962	WP_024179169.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FFJ14_RS11580 (2371269)	2371269..2371499	-	231	WP_000494244.1	YncJ family protein	-
FFJ14_RS11585 (2371721)	2371721..2371897	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FFJ14_RS11590 (2371943)	2371943..2372359	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FFJ14_RS11595 (2372438)	2372438..2373844	+	1407	WP_063113709.1	PLP-dependent aminotransferase family protein	-
FFJ14_RS11600 (2374089)	2374089..2375234	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
FFJ14_RS11605 (2375252)	2375252..2376262	+	1011	Protein_2280	ABC transporter ATP-binding protein	-
FFJ14_RS11610 (2376266)	2376266..2377207	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2366035..2367300	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T37988 WP_000813794.1 NZ_AP024126:2371721-2371897 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T37988 NZ_AP024126:2371721-2371897 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT37988 WP_001270286.1 NZ_AP024126:2371943-2372359 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT37988 NZ_AP024126:2371943-2372359 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB