Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1135530..1136148 Replicon chromosome
Accession NZ_AP024126
Organism Escherichia coli strain NS-NP030

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FFJ14_RS05435 Protein ID WP_001291435.1
Coordinates 1135530..1135748 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FFJ14_RS05440 Protein ID WP_000344800.1
Coordinates 1135774..1136148 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFJ14_RS05400 (1130819) 1130819..1131391 + 573 WP_063113571.1 YbaY family lipoprotein -
FFJ14_RS05405 (1131422) 1131422..1131733 - 312 WP_000409911.1 MGMT family protein -
FFJ14_RS05415 (1132112) 1132112..1132465 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FFJ14_RS05420 (1132507) 1132507..1134057 - 1551 WP_001352692.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FFJ14_RS05425 (1134221) 1134221..1134691 - 471 WP_000136192.1 YlaC family protein -
FFJ14_RS05430 (1134807) 1134807..1135358 - 552 WP_000102564.1 maltose O-acetyltransferase -
FFJ14_RS05435 (1135530) 1135530..1135748 - 219 WP_001291435.1 HHA domain-containing protein Toxin
FFJ14_RS05440 (1135774) 1135774..1136148 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FFJ14_RS05445 (1136694) 1136694..1139843 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
FFJ14_RS05450 (1139866) 1139866..1141059 - 1194 WP_063113572.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T37978 WP_001291435.1 NZ_AP024126:c1135748-1135530 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T37978 NZ_AP024126:c1135748-1135530 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT37978 WP_000344800.1 NZ_AP024126:c1136148-1135774 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT37978 NZ_AP024126:c1136148-1135774 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References