Detailed information of TA system TA037977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1101244..1102081
• Replicon: chromosome
• Accession: NZ_AP024126
• Organism: Escherichia coli strain NS-NP030

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: FFJ14_RS05265
• Protein ID: WP_000227784.1
• Coordinates: 1101244..1101786 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: FFJ14_RS05270
• Protein ID: WP_001297137.1
• Coordinates: 1101770..1102081 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFJ14_RS05245 (1096783)	1096783..1097694	-	912	WP_000705849.1	2-dehydropantoate 2-reductase	-
FFJ14_RS05250 (1097862)	1097862..1098353	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
FFJ14_RS05255 (1098481)	1098481..1099845	-	1365	WP_047083808.1	MFS transporter	-
FFJ14_RS05260 (1100253)	1100253..1101188	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
FFJ14_RS05265 (1101244)	1101244..1101786	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
FFJ14_RS05270 (1101770)	1101770..1102081	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
FFJ14_RS05275 (1102266)	1102266..1103156	-	891	WP_000971336.1	heme o synthase	-
FFJ14_RS05280 (1103168)	1103168..1103497	-	330	WP_100620279.1	cytochrome o ubiquinol oxidase subunit IV	-
FFJ14_RS05285 (1103497)	1103497..1104111	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FFJ14_RS05290 (1104101)	1104101..1106092	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FFJ14_RS05295 (1106114)	1106114..1107061	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T37977 WP_000227784.1 NZ_AP024126:c1101786-1101244 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T37977 NZ_AP024126:c1101786-1101244 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT37977 WP_001297137.1 NZ_AP024126:c1102081-1101770 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT37977 NZ_AP024126:c1102081-1101770 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3