Detailed information of TA system TA037970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 73097..73722
• Replicon: plasmid KS-NP019_P1
• Accession: NZ_AP024124
• Organism: Escherichia coli strain KS-NP019

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FFG67_RS25475
• Protein ID: WP_000911317.1
• Coordinates: 73324..73722 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q31SG2
• Locus tag: FFG67_RS25470
• Protein ID: WP_000450530.1
• Coordinates: 73097..73324 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFG67_RS25470 (73097)	73097..73324	+	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FFG67_RS25475 (73324)	73324..73722	+	399	WP_000911317.1	type II toxin-antitoxin system VapC family toxin	Toxin
FFG67_RS25480 (73731)	73731..75884	-	2154	WP_063113256.1	type IV conjugative transfer system coupling protein TraD	-
FFG67_RS25485 (75935)	75935..76672	-	738	WP_063113257.1	hypothetical protein	-
FFG67_RS25490 (76875)	76875..77606	-	732	WP_000850424.1	conjugal transfer complement resistance protein TraT	-
FFG67_RS25495 (77638)	77638..78135	-	498	WP_063113258.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cnf1 / hlyD / hlyB / hlyA / hlyC	1..127580	127580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T37970 WP_000911317.1 NZ_AP024124:73324-73722 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T37970 NZ_AP024124:73324-73722 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAATATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.59 Da
• Isoelectric Point: 4.7513

>AT37970 WP_000450530.1 NZ_AP024124:73097-73324 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT37970 NZ_AP024124:73097-73324 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: Q31SG2