Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4636069..4636687 Replicon chromosome
Accession NZ_AP024123
Organism Escherichia coli strain KS-NP019

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FFG67_RS22395 Protein ID WP_001291435.1
Coordinates 4636069..4636287 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FFG67_RS22400 Protein ID WP_000344800.1
Coordinates 4636313..4636687 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFG67_RS22360 (4631358) 4631358..4631930 + 573 WP_063113571.1 YbaY family lipoprotein -
FFG67_RS22365 (4631961) 4631961..4632272 - 312 WP_000409911.1 MGMT family protein -
FFG67_RS22375 (4632651) 4632651..4633004 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FFG67_RS22380 (4633046) 4633046..4634596 - 1551 WP_001352692.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FFG67_RS22385 (4634760) 4634760..4635230 - 471 WP_000136192.1 YlaC family protein -
FFG67_RS22390 (4635346) 4635346..4635897 - 552 WP_000102564.1 maltose O-acetyltransferase -
FFG67_RS22395 (4636069) 4636069..4636287 - 219 WP_001291435.1 HHA domain-containing protein Toxin
FFG67_RS22400 (4636313) 4636313..4636687 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FFG67_RS22405 (4637233) 4637233..4640382 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
FFG67_RS22410 (4640405) 4640405..4641598 - 1194 WP_063113572.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T37965 WP_001291435.1 NZ_AP024123:c4636287-4636069 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T37965 NZ_AP024123:c4636287-4636069 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT37965 WP_000344800.1 NZ_AP024123:c4636687-4636313 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT37965 NZ_AP024123:c4636687-4636313 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References