Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3786052..3786549 Replicon chromosome
Accession NZ_AP024123
Organism Escherichia coli strain KS-NP019

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FFG67_RS18185 Protein ID WP_001173343.1
Coordinates 3786376..3786549 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FFG67_RS18180 Protein ID WP_000398619.1
Coordinates 3786052..3786348 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFG67_RS18160 (3782783) 3782783..3783502 - 720 WP_000611288.1 two-component system response regulator DcuR -
FFG67_RS18165 (3783499) 3783499..3785130 - 1632 WP_001216487.1 sensor histidine kinase -
FFG67_RS18170 (3785311) 3785311..3785541 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
FFG67_RS18175 (3785553) 3785553..3785825 + 273 WP_000405649.1 GNAT family N-acetyltransferase -
FFG67_RS18180 (3786052) 3786052..3786348 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FFG67_RS18185 (3786376) 3786376..3786549 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FFG67_RS18190 (3786668) 3786668..3788185 - 1518 WP_001295087.1 lysine--tRNA ligase -
FFG67_RS18195 (3788422) 3788422..3789879 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T37961 WP_001173343.1 NZ_AP024123:3786376-3786549 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T37961 NZ_AP024123:3786376-3786549 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT37961 WP_000398619.1 NZ_AP024123:3786052-3786348 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT37961 NZ_AP024123:3786052-3786348 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References