Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3013867..3014498 Replicon chromosome
Accession NZ_AP024123
Organism Escherichia coli strain KS-NP019

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag FFG67_RS14660 Protein ID WP_001259385.1
Coordinates 3014223..3014498 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag FFG67_RS14655 Protein ID WP_000593554.1
Coordinates 3013867..3014226 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFG67_RS14625 (3008979) 3008979..3009452 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
FFG67_RS14630 (3009449) 3009449..3009730 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
FFG67_RS14635 (3009807) 3009807..3011165 + 1359 WP_063113578.1 PTS galactitol transporter subunit IIC -
FFG67_RS14640 (3011158) 3011158..3012666 + 1509 WP_001298718.1 FGGY-family carbohydrate kinase -
FFG67_RS14645 (3012656) 3012656..3012925 + 270 WP_000933060.1 HPr family phosphocarrier protein -
FFG67_RS14650 (3012957) 3012957..3013817 + 861 WP_001131783.1 class II aldolase -
FFG67_RS14655 (3013867) 3013867..3014226 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FFG67_RS14660 (3014223) 3014223..3014498 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
FFG67_RS14665 (3014571) 3014571..3015695 - 1125 WP_001216257.1 ABC transporter permease -
FFG67_RS14670 (3015695) 3015695..3018430 - 2736 WP_000149167.1 ribosome-associated ATPase/putative transporter RbbA -
FFG67_RS14675 (3018427) 3018427..3019494 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T37959 WP_001259385.1 NZ_AP024123:c3014498-3014223 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T37959 NZ_AP024123:c3014498-3014223 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT37959 WP_000593554.1 NZ_AP024123:c3014226-3013867 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT37959 NZ_AP024123:c3014226-3013867 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References