Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2403532..2404186 Replicon chromosome
Accession NZ_AP024123
Organism Escherichia coli strain KS-NP019

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag FFG67_RS11585 Protein ID WP_000244772.1
Coordinates 2403532..2403939 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FFG67_RS11590 Protein ID WP_000354046.1
Coordinates 2403920..2404186 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFG67_RS11565 (2399489) 2399489..2401222 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
FFG67_RS11570 (2401228) 2401228..2401938 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FFG67_RS11575 (2401963) 2401963..2402859 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FFG67_RS11580 (2402971) 2402971..2403492 + 522 WP_001055874.1 flavodoxin FldB -
FFG67_RS11585 (2403532) 2403532..2403939 - 408 WP_000244772.1 protein YgfX Toxin
FFG67_RS11590 (2403920) 2403920..2404186 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FFG67_RS11595 (2404429) 2404429..2405409 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FFG67_RS11600 (2405662) 2405662..2406642 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
FFG67_RS11605 (2406765) 2406765..2407424 - 660 WP_000250274.1 hemolysin III family protein -
FFG67_RS11610 (2407588) 2407588..2407899 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2405662..2406642 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T37957 WP_000244772.1 NZ_AP024123:c2403939-2403532 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T37957 NZ_AP024123:c2403939-2403532 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT37957 WP_000354046.1 NZ_AP024123:c2404186-2403920 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT37957 NZ_AP024123:c2404186-2403920 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References