Detailed information of TA system TA037955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1959284..1959909
• Replicon: chromosome
• Accession: NZ_AP024123
• Organism: Escherichia coli strain KS-NP019

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FFG67_RS09460
• Protein ID: WP_000911330.1
• Coordinates: 1959284..1959682 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FFG67_RS09465
• Protein ID: WP_000450524.1
• Coordinates: 1959682..1959909 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFG67_RS09440 (1955191)	1955191..1955391	+	201	WP_000383836.1	YpfN family protein	-
FFG67_RS09445 (1955472)	1955472..1956170	-	699	WP_000679812.1	esterase	-
FFG67_RS09450 (1956244)	1956244..1958259	-	2016	WP_033885019.1	tRNA cytosine(34) acetyltransferase TmcA	-
FFG67_RS09455 (1958274)	1958274..1959137	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
FFG67_RS09460 (1959284)	1959284..1959682	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
FFG67_RS09465 (1959682)	1959682..1959909	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FFG67_RS09470 (1960063)	1960063..1960776	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FFG67_RS09475 (1960989)	1960989..1962023	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FFG67_RS09480 (1962040)	1962040..1962918	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FFG67_RS09485 (1963064)	1963064..1963636	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FFG67_RS09490 (1963636)	1963636..1964106	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T37955 WP_000911330.1 NZ_AP024123:c1959682-1959284 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T37955 NZ_AP024123:c1959682-1959284 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT37955 WP_000450524.1 NZ_AP024123:c1959909-1959682 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT37955 NZ_AP024123:c1959909-1959682 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33