Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 659006..659644 Replicon chromosome
Accession NZ_AP024123
Organism Escherichia coli strain KS-NP019

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FFG67_RS03170 Protein ID WP_000813794.1
Coordinates 659006..659182 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FFG67_RS03175 Protein ID WP_001270286.1
Coordinates 659228..659644 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFG67_RS03150 (654625) 654625..655800 - 1176 WP_063103682.1 BenE family transporter YdcO -
FFG67_RS03155 (655892) 655892..656428 + 537 WP_063103683.1 DNA-binding transcriptional regulator SutR -
FFG67_RS03160 (656501) 656501..658462 + 1962 WP_024179169.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FFG67_RS03165 (658554) 658554..658784 - 231 WP_000494244.1 YncJ family protein -
FFG67_RS03170 (659006) 659006..659182 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FFG67_RS03175 (659228) 659228..659644 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FFG67_RS03180 (659723) 659723..661129 + 1407 WP_063113709.1 PLP-dependent aminotransferase family protein -
FFG67_RS03185 (661374) 661374..662519 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
FFG67_RS03190 (662537) 662537..663547 + 1011 Protein_630 ABC transporter ATP-binding protein -
FFG67_RS03195 (663551) 663551..664492 + 942 WP_137516484.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 653320..654585 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T37943 WP_000813794.1 NZ_AP024123:659006-659182 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T37943 NZ_AP024123:659006-659182 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT37943 WP_001270286.1 NZ_AP024123:659228-659644 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT37943 NZ_AP024123:659228-659644 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References