Detailed information of TA system TA037932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 29441..30000
• Replicon: plasmid JML285_P2
• Accession: NZ_AP024116
• Organism: Escherichia coli strain JML285

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: FFF71_RS24700
• Protein ID: WP_000421257.1
• Coordinates: 29725..30000 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A5I3EQ05
• Locus tag: FFF71_RS24695
• Protein ID: WP_001674495.1
• Coordinates: 29441..29725 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFF71_RS24675 (24454)	24454..25362	-	909	WP_003155746.1	TniB family NTP-binding protein	-
FFF71_RS24680 (25365)	25365..27044	-	1680	WP_000179844.1	DDE-type integrase/transposase/recombinase	-
FFF71_RS24685 (27119)	27119..27184	-	66	Protein_25	EAL domain-containing protein	-
FFF71_RS24690 (27478)	27478..29055	+	1578	WP_001323889.1	PAS domain-containing methyl-accepting chemotaxis protein	-
FFF71_RS24695 (29441)	29441..29725	+	285	WP_001674495.1	ribbon-helix-helix domain-containing protein	Antitoxin
FFF71_RS24700 (29725)	29725..30000	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FFF71_RS24705 (30148)	30148..31116	+	969	WP_000654812.1	IS5-like element IS903B family transposase	-
FFF71_RS24710 (31174)	31174..31287	+	114	Protein_30	colicin-like pore-forming protein	-
FFF71_RS24715 (31309)	31309..31644	-	336	WP_001080730.1	colicin 1A immunity protein	-
FFF71_RS24720 (31773)	31773..32120	+	348	WP_016230895.1	DUF6404 family protein	-
FFF71_RS24725 (32138)	32138..32728	-	591	WP_000194548.1	hypothetical protein	-
FFF71_RS24730 (32728)	32728..32985	-	258	WP_000371887.1	hypothetical protein	-
FFF71_RS24735 (33555)	33555..33869	+	315	WP_001302628.1	hypothetical protein	-
FFF71_RS24740 (33931)	33931..34701	+	771	WP_001499400.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / tet(M)	-	1..95339	95339
	-	flank	IS/Tn	-	-	30193..31116	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T37932 WP_000421257.1 NZ_AP024116:29725-30000 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T37932 NZ_AP024116:29725-30000 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT37932 WP_001674495.1 NZ_AP024116:29441-29725 [Escherichia coli]
MQMKNNSAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT37932 NZ_AP024116:29441-29725 [Escherichia coli]
ATGCAAATGAAAAACAATAGTGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A5I3EQ05