Detailed information of TA system TA037927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1981..2540
• Replicon: plasmid JML285_P1
• Accession: NZ_AP024115
• Organism: Escherichia coli strain JML285

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: FFF71_RS23955
• Protein ID: WP_000421259.1
• Coordinates: 2265..2540 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: FFF71_RS23950
• Protein ID: WP_001178087.1
• Coordinates: 1981..2265 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFF71_RS23945 (46)	46..1077	+	1032	WP_000907852.1	plasmid replication initiator RepA	-
FFF71_RS23950 (1981)	1981..2265	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
FFF71_RS23955 (2265)	2265..2540	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FFF71_RS23960 (2646)	2646..2849	+	204	Protein_3	hypothetical protein	-
FFF71_RS23965 (3351)	3351..5150	-	1800	Protein_4	Tn3 family transposase	-
FFF71_RS23970 (5245)	5245..6675	+	1431	Protein_5	IS4-like element IS50R family transposase	-
FFF71_RS23975 (6704)	6704..7498	+	795	WP_000572405.1	aminoglycoside O-phosphotransferase APH(3')-IIa	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-IIa / sul1 / qacE / aph(3'')-Ib / aph(6)-Id	-	1..109993	109993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T37927 WP_000421259.1 NZ_AP024115:2265-2540 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T37927 NZ_AP024115:2265-2540 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT37927 WP_001178087.1 NZ_AP024115:1981-2265 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT37927 NZ_AP024115:1981-2265 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625