Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4515657..4516154 Replicon chromosome
Accession NZ_AP024114
Organism Escherichia coli strain JML285

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FFF71_RS21730 Protein ID WP_001173343.1
Coordinates 4515657..4515830 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FFF71_RS21735 Protein ID WP_000398619.1
Coordinates 4515858..4516154 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFF71_RS21720 (4512327) 4512327..4513784 + 1458 WP_040090433.1 dipeptide/tripeptide permease DtpC -
FFF71_RS21725 (4514021) 4514021..4515538 + 1518 WP_001295074.1 lysine--tRNA ligase -
FFF71_RS21730 (4515657) 4515657..4515830 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FFF71_RS21735 (4515858) 4515858..4516154 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FFF71_RS21740 (4516381) 4516381..4516653 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
FFF71_RS21745 (4516665) 4516665..4516895 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
FFF71_RS21750 (4517076) 4517076..4518707 + 1632 WP_001216487.1 sensor histidine kinase -
FFF71_RS21755 (4518704) 4518704..4519423 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4482372..4516154 33782


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T37925 WP_001173343.1 NZ_AP024114:c4515830-4515657 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T37925 NZ_AP024114:c4515830-4515657 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT37925 WP_000398619.1 NZ_AP024114:c4516154-4515858 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT37925 NZ_AP024114:c4516154-4515858 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References