Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4435410..4436005 Replicon chromosome
Accession NZ_AP024114
Organism Escherichia coli strain JML285

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag FFF71_RS21315 Protein ID WP_000239579.1
Coordinates 4435655..4436005 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag FFF71_RS21310 Protein ID WP_001223208.1
Coordinates 4435410..4435661 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFF71_RS21300 (4431074) 4431074..4434853 + 3780 WP_040089791.1 autotransporter assembly complex protein TamB -
FFF71_RS21305 (4434856) 4434856..4435197 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
FFF71_RS21310 (4435410) 4435410..4435661 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
FFF71_RS21315 (4435655) 4435655..4436005 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
FFF71_RS21320 (4436085) 4436085..4436615 - 531 WP_000055072.1 inorganic diphosphatase -
FFF71_RS21325 (4436925) 4436925..4437881 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
FFF71_RS21330 (4438021) 4438021..4439523 + 1503 WP_000205810.1 sugar ABC transporter ATP-binding protein -
FFF71_RS21335 (4439537) 4439537..4440559 + 1023 WP_024251350.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T37923 WP_000239579.1 NZ_AP024114:4435655-4436005 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T37923 NZ_AP024114:4435655-4436005 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT37923 WP_001223208.1 NZ_AP024114:4435410-4435661 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT37923 NZ_AP024114:4435410-4435661 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References