Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3635082..3635700 Replicon chromosome
Accession NZ_AP024114
Organism Escherichia coli strain JML285

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FFF71_RS17515 Protein ID WP_001291435.1
Coordinates 3635482..3635700 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FFF71_RS17510 Protein ID WP_000344800.1
Coordinates 3635082..3635456 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFF71_RS17500 (3630171) 3630171..3631364 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FFF71_RS17505 (3631387) 3631387..3634536 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
FFF71_RS17510 (3635082) 3635082..3635456 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FFF71_RS17515 (3635482) 3635482..3635700 + 219 WP_001291435.1 HHA domain-containing protein Toxin
FFF71_RS17520 (3635871) 3635871..3636422 + 552 WP_000102564.1 maltose O-acetyltransferase -
FFF71_RS17525 (3636538) 3636538..3637008 + 471 WP_000136192.1 YlaC family protein -
FFF71_RS17530 (3637172) 3637172..3638722 + 1551 WP_040089986.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FFF71_RS17535 (3638764) 3638764..3639117 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FFF71_RS17545 (3639496) 3639496..3639807 + 312 WP_000409911.1 MGMT family protein -
FFF71_RS17550 (3639838) 3639838..3640410 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T37919 WP_001291435.1 NZ_AP024114:3635482-3635700 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T37919 NZ_AP024114:3635482-3635700 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT37919 WP_000344800.1 NZ_AP024114:3635082-3635456 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT37919 NZ_AP024114:3635082-3635456 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References