Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2536849..2537487 Replicon chromosome
Accession NZ_AP024114
Organism Escherichia coli strain JML285

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FFF71_RS12240 Protein ID WP_000813794.1
Coordinates 2537311..2537487 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FFF71_RS12235 Protein ID WP_001270286.1
Coordinates 2536849..2537265 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFF71_RS12215 (2532001) 2532001..2532942 - 942 WP_001251313.1 ABC transporter permease -
FFF71_RS12220 (2532943) 2532943..2533956 - 1014 WP_001542893.1 ABC transporter ATP-binding protein -
FFF71_RS12225 (2533974) 2533974..2535119 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FFF71_RS12230 (2535364) 2535364..2536770 - 1407 WP_000760663.1 PLP-dependent aminotransferase family protein -
FFF71_RS12235 (2536849) 2536849..2537265 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FFF71_RS12240 (2537311) 2537311..2537487 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FFF71_RS12245 (2537709) 2537709..2537939 + 231 WP_000494244.1 YncJ family protein -
FFF71_RS12250 (2538031) 2538031..2539992 - 1962 WP_001442195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FFF71_RS12255 (2540065) 2540065..2540601 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FFF71_RS12260 (2540693) 2540693..2541868 + 1176 WP_001583501.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2541908..2543056 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T37917 WP_000813794.1 NZ_AP024114:c2537487-2537311 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T37917 NZ_AP024114:c2537487-2537311 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT37917 WP_001270286.1 NZ_AP024114:c2537265-2536849 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT37917 NZ_AP024114:c2537265-2536849 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References