Detailed information of TA system TA037907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1480830..1481455
• Replicon: chromosome
• Accession: NZ_AP024114
• Organism: Escherichia coli strain JML285

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FFF71_RS07245
• Protein ID: WP_000911330.1
• Coordinates: 1481057..1481455 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FFF71_RS07240
• Protein ID: WP_000450524.1
• Coordinates: 1480830..1481057 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFF71_RS07215 (1476633)	1476633..1477103	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FFF71_RS07220 (1477103)	1477103..1477675	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FFF71_RS07225 (1477821)	1477821..1478699	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FFF71_RS07230 (1478716)	1478716..1479750	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
FFF71_RS07235 (1479963)	1479963..1480676	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FFF71_RS07240 (1480830)	1480830..1481057	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FFF71_RS07245 (1481057)	1481057..1481455	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
FFF71_RS07250 (1481602)	1481602..1482465	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
FFF71_RS07255 (1482480)	1482480..1484495	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
FFF71_RS07260 (1484569)	1484569..1485267	+	699	WP_000679812.1	esterase	-
FFF71_RS07265 (1485348)	1485348..1485548	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T37907 WP_000911330.1 NZ_AP024114:1481057-1481455 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T37907 NZ_AP024114:1481057-1481455 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT37907 WP_000450524.1 NZ_AP024114:1480830-1481057 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT37907 NZ_AP024114:1480830-1481057 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33