Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1003762..1004416 Replicon chromosome
Accession NZ_AP024114
Organism Escherichia coli strain JML285

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FFF71_RS04895 Protein ID WP_000244777.1
Coordinates 1004009..1004416 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FFF71_RS04890 Protein ID WP_000354046.1
Coordinates 1003762..1004028 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFF71_RS04865 (998931) 998931..999674 + 744 WP_137515324.1 SDR family oxidoreductase -
FFF71_RS04870 (999731) 999731..1001164 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
FFF71_RS04875 (1001209) 1001209..1001520 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
FFF71_RS04880 (1001684) 1001684..1002343 + 660 WP_000250274.1 hemolysin III family protein -
FFF71_RS04885 (1002539) 1002539..1003519 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FFF71_RS04890 (1003762) 1003762..1004028 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FFF71_RS04895 (1004009) 1004009..1004416 + 408 WP_000244777.1 protein YgfX Toxin
FFF71_RS04900 (1004456) 1004456..1004977 - 522 WP_001055874.1 flavodoxin FldB -
FFF71_RS04905 (1005089) 1005089..1005985 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FFF71_RS04910 (1006010) 1006010..1006720 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FFF71_RS04915 (1006726) 1006726..1008459 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T37905 WP_000244777.1 NZ_AP024114:1004009-1004416 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T37905 NZ_AP024114:1004009-1004416 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT37905 WP_000354046.1 NZ_AP024114:1003762-1004028 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT37905 NZ_AP024114:1003762-1004028 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References