Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 108816..109459 Replicon plasmid ECSC054_P1
Accession NZ_AP024113
Organism Escherichia coli strain ECSC054

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag FE985_RS25880 Protein ID WP_001034044.1
Coordinates 109043..109459 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag FE985_RS25875 Protein ID WP_001261286.1
Coordinates 108816..109046 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE985_RS25855 (105379) 105379..106134 - 756 WP_000852146.1 replication initiation protein RepE -
FE985_RS25860 (106856) 106856..107662 - 807 WP_000016970.1 site-specific integrase -
FE985_RS25865 (107663) 107663..107968 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
FE985_RS25870 (107970) 107970..108188 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
FE985_RS25875 (108816) 108816..109046 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FE985_RS25880 (109043) 109043..109459 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
FE985_RS25885 (109534) 109534..111099 + 1566 WP_001128474.1 AAA family ATPase -
FE985_RS25890 (111084) 111084..112106 + 1023 WP_001563708.1 hypothetical protein -
FE985_RS25900 (113099) 113099..113482 + 384 WP_024191435.1 fluoride efflux transporter CrcB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B iucA / iucB / iucC / iucD / iutA 1..126311 126311
- flank IS/Tn - - 112360..112626 266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T37899 WP_001034044.1 NZ_AP024113:109043-109459 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T37899 NZ_AP024113:109043-109459 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT37899 WP_001261286.1 NZ_AP024113:108816-109046 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT37899 NZ_AP024113:108816-109046 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References