Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 107663..108188 Replicon plasmid ECSC054_P1
Accession NZ_AP024113
Organism Escherichia coli strain ECSC054

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FE985_RS25865 Protein ID WP_001159871.1
Coordinates 107663..107968 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag FE985_RS25870 Protein ID WP_000813630.1
Coordinates 107970..108188 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE985_RS25850 (103625) 103625..104791 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
FE985_RS25855 (105379) 105379..106134 - 756 WP_000852146.1 replication initiation protein RepE -
FE985_RS25860 (106856) 106856..107662 - 807 WP_000016970.1 site-specific integrase -
FE985_RS25865 (107663) 107663..107968 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FE985_RS25870 (107970) 107970..108188 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FE985_RS25875 (108816) 108816..109046 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
FE985_RS25880 (109043) 109043..109459 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
FE985_RS25885 (109534) 109534..111099 + 1566 WP_001128474.1 AAA family ATPase -
FE985_RS25890 (111084) 111084..112106 + 1023 WP_001563708.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B iucA / iucB / iucC / iucD / iutA 1..126311 126311
- flank IS/Tn - - 112360..112626 266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T37898 WP_001159871.1 NZ_AP024113:c107968-107663 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T37898 NZ_AP024113:c107968-107663 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT37898 WP_000813630.1 NZ_AP024113:c108188-107970 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT37898 NZ_AP024113:c108188-107970 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References