Detailed information of TA system TA037894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 54891..55516
• Replicon: plasmid ECSC054_P1
• Accession: NZ_AP024113
• Organism: Escherichia coli strain ECSC054

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FE985_RS25545
• Protein ID: WP_000911333.1
• Coordinates: 55118..55516 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: FE985_RS25540
• Protein ID: WP_000450520.1
• Coordinates: 54891..55118 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE985_RS25540 (54891)	54891..55118	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FE985_RS25545 (55118)	55118..55516	+	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FE985_RS25550 (55525)	55525..57678	-	2154	WP_039719975.1	type IV conjugative transfer system coupling protein TraD	-
FE985_RS25555 (57931)	57931..58662	-	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
FE985_RS25560 (58676)	58676..59185	-	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	iucA / iucB / iucC / iucD / iutA	1..126311	126311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T37894 WP_000911333.1 NZ_AP024113:55118-55516 [Escherichia coli]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T37894 NZ_AP024113:55118-55516 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT37894 WP_000450520.1 NZ_AP024113:54891-55118 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT37894 NZ_AP024113:54891-55118 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2